949 research outputs found
Elastic behaviour of the carotid artery in intact spontaneously hypertensive rats
Intact spontaneously hypertensive rats (SHR) were studied to assess the effect of prolonged antihypertensive treatment on the elastic behaviour of the external carotid artery. Thirty-week-old SHR received the ACE inhibitor captopril, the ateriolar dilator hydralazine or their vehicle for 6 weeks. These rats were compared to normotensive, vehicle treated WKY rats. The internal diameter of the carotid artery was measured continuously in halothane-anaesthetized rats using an echo-tracking device, and intra-arterial pressure was also monitored continuously, on the controlateral side. Captopril- and hydralazinetreated SHR as well as normotensive controls had similar blood pressure values. No significant shift in the distensibility-pressure curves was observed among vehicle-treated SHR and WKY rats or the SHR which had received captopril or hydralazine. Histological examination of the carotid artery fixed ex vivo with paraformaldehyde showed a significant increase in cross-sectional area in vehicle-treated SHR as compared to their normotensive counterparts. These results therefore suggest that the elastic behaviour of elastic arteries is not necessarily altered by the structural changes developing in response to hypertensio
Microsatellite instability and defects in mismatch repair proteins: a new aetiology for Sertoli cell‐only syndrome
Microsatellite instability is characteristic of certain types of cancer, and is present in rodents lacking specific DNA mismatch repair proteins. These azoospermic mice exhibit spermatogenic defects similar to some human testicular failure patients. Therefore, we hypothesized that microsatellite instability due to deficiencies in mismatch repair genes might be an unrecognized aetiology of human testicular failure. Because these azoospermic patients are candidates for testicular sperm extraction and ICSI, transmission of mismatch repair defects to the offspring is possible. Seven microsatellite loci were analysed for instability in specimens from 41 testicular failure patients and 20 controls. Blood and testicular DNA were extracted from patient and control specimens, and amplified by PCR targeting seven microsatellite loci. DNA fragment length was analysed with an ABI Prism 310 Genotyping Machine and GeneScan software. Immunohistochemistry was performed on paraffinized testis biopsy sections and cultured testicular fibroblasts from each patient to determine if expression of the mismatch repair proteins hMSH2 and hMLH1 was normal in both somatic and germline cells. Results demonstrate that microsatellite instability and DNA mismatch repair protein defects are present in some azoospermic men, predominantly in Sertoli cell‐only patients (P < 0.01 and P < 0.05 respectively). This provides evidence of a previously unrecognized aetiology of testicular failure that may be associated with cancer predispositio
A graph-based modelling methodology for high-pressure networks applied on waterjet machining
This paper proposes a graph-based methodology that models high-pressure networks of varioustopologies. Therefore, a mathematical modelling of a supply network for waterjet machining will be introduced. High-pressure components are assigned to homogeneous segments, each representing a local pressure state as a differential equation. Segments are subsequently interconnected along the fluid flow path as an algebraic equation that allocates a fluid flow to the interconnections, resulting in a lumped parameter model. For this purpose, a graph network description has been used to approximate the spatially distributed high-pressure system. In this way, the proposed methodology offers a flexible modelling to cope with different network topologies. Moreover, a variable fluid compressibility has also been introduced so that a wide operating range can be included. This modelling methodology has been applied to a supply network for waterjet machining. The resulting mathematical model has been verified by measurements from a test bench with a pressure range of 100 to 400 MPa. It was shown that a variable fluid compressibility improves the model’s accuracy and that modelling errors can be reduced in comparison to other existing methodologies
Transport regimes of cold gases in a two-dimensional anisotropic disorder
We numerically study the dynamics of cold atoms in a two-dimensional
disordered potential. We consider an anisotropic speckle potential and focus on
the classical regime, which is relevant to some recent experiments. First, we
study the behavior of particles with a fixed energy and identify different
transport regimes. For low energy, the particles are classically localized due
to the absence of a percolating cluster. For high energy, the particles undergo
normal diffusion and we show that the diffusion constants scale algebraically
with the particle energy, with an anisotropy factor which significantly differs
from that of the disordered potential. For intermediate energy, we find a
transient sub-diffusive regime, which is relevant to the time scale of typical
experiments. Second, we study the behavior of a cold-atomic gas with an
arbitrary energy distribution, using the above results as a groundwork. We show
that the density profile of the atomic cloud in the diffusion regime is
strongly peaked and, in particular, that it is not Gaussian. Its behavior at
large distances allows us to extract the energy-dependent diffusion constants
from experimental density distributions. For a thermal cloud released into the
disordered potential, we show that our numerical predictions are in agreement
with experimental findings. Not only does this work give insights to recent
experimental results, but it may also serve interpretation of future
experiments searching for deviation from classical diffusion and traces of
Anderson localization.Comment: 19 pages, 16 figure
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